Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1

A cyclodextrin glucanotransferase (CGTase) was successively purified by ammonium sulphate precipitation, and affinity chromatography on a-CD (epoxy)-Sepharose 6B column. The specific activity of the CGTase was increased approximately 2200-fold, from 8.43 U/mg protein to 18,866 U/mg protein. SDS-PAGE showed that the purified CGTase was homogeneous and the molecular weight of the purified CGTase was about 75 kDa. The molecular weight of the enzyme that was estimated by gel filtration under native condition was 79 kDa. This has indicated that Bacillus sp. G1 CGTase is a monomeric protein. The isoelectric point (pI) of the enzyme was about 8.8. Characterization of the enzyme exhibited optimum pH and temperature of 6.0 and 60 8C, respectively. The enzyme was stable from pH 7.0 to 9.0 and retained its high activity up to 60 8C. However, in the presence of 20 mM Ca2+, the purified CGTase is able to prolong its thermal stability up to 70 8C. CGTase was strongly inhibited by ZnSO4, CuSO4, CoCl2, FeSO4, FeCl3 and EDTA. Km and Vmax for the purified enzyme were 0.15 mg/ml and 60.39 mg bcyclodextrin/( ml min), respectively, with soluble starch as substrate. In cyclodextrin production, tapioca starch was found to be the best substrate used to produce CDs. The enzyme produced g- and b-CD in the ratio of 0.11:0.89 after 24 h incubation at 60 8C, without the presence of any selective agents

Characterization of flow rate and Heat Loss in Heating, Ventilation and Air Conditioning (HVAC) Duct System for Office Building

A building is an assemblage that is firmly attached to the ground and provides the performance of human activities and need to be considered in the daily operation in that building. The improvements in building performance are focused on improving the energy efficiency of buildings. This is approach by designing heating, ventilation and air conditioning (HVAC) duct system due to one of the most utilized energy in maintaining building performance and environment. The objectives of this research is to calculate the air (CFM) supply in office building, to characterize the velocity and head loss in a round and rectangular HVAC ducting system at various duct thickness and to optimize the thickness of the duct in HVAC system according to ASHRAE Standard. The increasing of velocity in duct system shows the increasing of head loss. The round duct design gives the lowest velocity and head loss in HVAC system approximately around 9.35% as compared to rectangular duct at 0.06 inches thickness. Hence, the trends of the head loss and duct thickness has influenced in reducing noise in HVAC duct system in order to select the best design concepts which is round shape design

Stress intensity factor for multiple inclined or curved cracks problem in circular positions in plane elasticity

The problems of multiple inclined or curved cracks in circular positions is treated by using the hypersingular integral equation method. The cracks center are placed at the edge of a virtual circle with radius R. The first crack is fixed on the x-axis while the second crack is located on the boundary of a circle with the varying angle, θ. A system of hypersingular integral equations is formulated and solved numerically for the stress intensity factor (SIF). Numerical examples demonstrate the effect of interaction between two cracks in circular positions are given. It is found that, the severity at the second crack tips are significant when the ratio length of the second to the first crack is small and it is placed at a small angle of θ

Straight cracks in circular positions in plane elasticity

A system of hypersingular integral equation for the multiple straight cracks in a circular position in plane elasticity is formulated and presented. The center of the cracks are placed at the edge of a circle with radius R. The second crack in this problem is located in a different position based on the varying angle, θ. The sraight cracks problem is reduced to a system of hypersingular integral equations by using the method of complex potential. With the help of particular quadrature rules, the unknown coefficients are solved numerically from the resulting system of hypersingular integral equations. The obtained unknown coefficients are then can be used for determining the stress intensity factor (SIF). To prove that the suggested method can be used to solve more complicated model cases of the cracks in circular positions, examples are given to demonstrate the behaviour of SIF for different crack positions

An Overview of Spark Ignition Engine Operating on Lower-Higher Molecular Mass Alcohol Blended Gasoline Fuels

This paper reviews the utilization of lower and higher molecular weight alcohols as fuel for spark ignition engine. As an alternative fuel for spark ignition engine, alcohol is widely accepted as comparable to gasolin. It is due to its ability that can be produced from biological matter through the current available and new processes. Moreover, alcohol is also considered as fuel additive due to its physical and chemical properties compatible with the requirements of modern engines. The objective of this paper is to provide an overview of these fuels by highlighting on the fuel properties and spark ignition engine responses. The first part of this review explains the important of alcohol fuel properties related to the engine performance and emissions, and the difference of these properties for each type of alcohol. The second part discusses recent advancements in research involving lower and higher molecular weight alcohols mainly responses from spark ignition engine

Salford postgraduate annual research conference (SPARC) 2012 proceedings

These proceedings bring together a selection of papers from the 2012 Salford Postgraduate Annual Research Conference (SPARC). They reflect the breadth and diversity of research interests showcased at the conference, at which over 130 researchers from Salford, the North West and other UK universities presented their work. 21 papers are collated here from the humanities, arts, social sciences, health, engineering, environment and life sciences, built environment and business

A sensitive acoustic sensor using fiber bragg grating based on strain compensation

An acoustic sensitivity single ring fiber sensor with enhanced pressure sensitivity is designed by using Fiber Bragg Grating (FBG). Measurement sensitivity of the this fiber sensor is 0.3714 με/Hz. This sensor exhibited a good acoustic sensitivity, showing that the sensor has good sensing properties for the detection of the acoustic signals

Curvature homogeneous spacelike Jordan Osserman pseudo-Riemannian manifolds

Let s be at least 2. We construct Ricci flat pseudo-Riemannian manifolds of signature (2s,s) which are not locally homogeneous but whose curvature tensors never the less exhibit a number of important symmetry properties. They are curvature homogeneous; their curvature tensor is modeled on that of a local symmetric space. They are spacelike Jordan Osserman with a Jacobi operator which is nilpotent of order 3; they are not timelike Jordan Osserman. They are k-spacelike higher order Jordan Osserman for $2\le k\le s$; they are k-timelike higher order Jordan Osserman for $s+2\le k\le 2s$, and they are not k timelike higher order Jordan Osserman for $2\le s\le s+1$.Comment: Update bibliography, fix minor misprint